The MagniSort® Mouse NK cell Enrichment Kit has been tested by magnetic cell separation followed by flow cytometric analysis of mouse splenocytes. A test is defined as the amount of antibody or beads to be used to stain 1×107 cells in 100 ?L. Page 2 of 2, With these two components, the BD IMag™ Mouse NK Cell Enrichment Set – DM avoids the inadvertent activation of the enriched NK cells by using reagents that do not directly bind to those NK cells . This set has been optimized for use with the BD IMag™ Cell Separation Magnet, and contains sufficient reagents to label 10^9 leukocytes.
The NK Cell Isolation Kit was developed for the isolation of untouched NK cells from single- cell suspensions of murine spleen. Non-NK cells , i.e. T cells , dendritic cells , B cells , granulocytes, macrophages, and erythroid cells are magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure unlabeled NK cells is achieved by …
1/1/2020 · Hydrodynamic pSushi-IL15-Apo delivery in Rag1 ?/? mice results in approximately a fivefold NK cell expansion with 40% of total NK cells in the spleen (Fig. 2A). After negative magnetic selection of NK cells using the mouse NK Cell Isolation Kit (Miltenyi), a purity of between 85% and 90% is accomplished (Fig. 2B) and an average of 5 million NK cells per mouse can be recovered per spleen …
